High purity trans-resveratrol economic production system

This technology is about a method of producing trans-resveratrol, a high value-added product, by using magnetic photosynthetic membrane vesicles together with immobilized biosynthetic enzymes.

Existing trans-resveratrol production methods had limitations such as low yield, complicated purification, and high cost. This technology proposes an innovative in vitro production method using magnetic photosynthetic cell membrane vesicles and immobilized enzymes to increase economic efficiency through repeated reuse of high-cost coenzymes and reduce production costs through efficient recovery and reuse of enzymes. In addition, the final product, trans-resveratrol, can be easily separated in the form of a high-purity precipitate, simplifying the purification process, and providing high-quality resveratrol through stereo-specific production, improving the possibility of industrial mass production of high-value resveratrol.

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Key Features:

• Enzyme-based synthesis: Using 4-coumaric acid and malonic acid as starting materials, trans-resveratrol is synthesized through three major enzymes (4-coumaric acid-Coenzyme A ligase, malonyl Coenzyme A synthetase, and stilbene synthase).
• Coenzyme (ATP) regeneration system: Photosynthetic cell membrane vesicles absorb light energy, continuously converting the ATP consumed during the reaction from ADP. Regeneration
• Magnetic-based recovery: Magnetic particles are contained inside the photosynthetic cell membrane vesicles, and after the reaction, they are simply separated from the reaction solution with a magnet and reused.
• The magnetic photosynthetic cell membrane vesicles regenerate ATP from ADP and Pi using light energy and resupply it to the reaction

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This technology was developed through the cutting-edge convergence technology development research project of the Ministry of Science, ICT and Future Planning.

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A precise object tracking system that excels even in low-quality images

This technology relates to an object tracking method and object tracking system. It relates to an object tracking method and device that is robust to the environment by obtaining multiple histograms for at least two features of a target object, selecting the one feature with the maximum cost among them, and applying a particle filter to track the object.

To overcome these limitations, this technology presents a particle filter-based object tracking technique that combines multiple histograms and Minimax estimation techniques. Accurate tracking is possible in any environment by analyzing two or more feature information, selecting the optimal feature, and calculating the particle weight.

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Key Features:

• A new object tracking method that combines the Minimax estimation technique with the Monte Carlo sampling (particle filter) method
• Simultaneously generates multiple histograms for multiple features such as the color (RGB, HSV) and shape (HOG) of the object
• Calculate the Bhattacharya distance with the reference model to obtain the cost, and select the one feature with the largest cost (maximum distance) among them
• Track lighting, shade, brightness changes, etc. through the Minimax technique When an interfering factor occurs, maintain stable tracking performance by using the feature most affected by the factor as a standard

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A precise object tracking system that excels even in low-quality images

This technology relates to an object tracking method and object tracking system. It relates to an object tracking method and device that is robust to the environment by obtaining multiple histograms for at least two features of a target object, selecting the one feature with the maximum cost among them, and applying a particle filter to track the object.

To overcome these limitations, this technology presents a particle filter-based object tracking technique that combines multiple histograms and Minimax estimation techniques. Accurate tracking is possible in any environment by analyzing two or more feature information, selecting the optimal feature, and calculating the particle weight.

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Key Features:

• A new object tracking method that combines the Minimax estimation technique with the Monte Carlo sampling (particle filter) method
• Simultaneously generates multiple histograms for multiple features such as the color (RGB, HSV) and shape (HOG) of the object
• Calculate the Bhattacharya distance with the reference model to obtain the cost, and select the one feature with the largest cost (maximum distance) among them
• Track lighting, shade, brightness changes, etc. through the Minimax technique When an interfering factor occurs, maintain stable tracking performance by using the feature most affected by the factor as a standard

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Computing resource saving blockchain transaction verification technology

This technology relates to a blockchain-based transaction verification system and method that can increase the efficiency of computing resources while maintaining the integrity of transaction verification by randomly assigning block verification rights to a small number of nodes using a block hash-based algorithm.

The existing blockchain transaction verification method had the problem of severe waste of computing resources because all nodes participated. To solve this inefficiency, this technology utilizes a block hash-based algorithm to randomly assign block verification authority to only a small number of nodes, thereby reducing computing resource usage by 94% while maintaining transaction integrity. At the same time, it has the effect of guaranteeing integrity even if a small number of nodes verify the transaction.

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Key Features:

• Only a small number of nodes randomly assigned block verification authority verify transactions
• Verification authority is assigned through an unpredictable algorithm using the hash value of the previous block and its own node ID
• Since verification authority changes in real time and unpredictably every time a block is created, integrity is secured by blocking the possibility of collusion and attack by a small number of nodes
• Only a small number of nodes perform verification to reduce resource consumption of the entire network and operate efficiently

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Biomimetic fiber network for cell tissue regeneration scaffold

This technology relates to a method of manufacturing a fiber network of extracellular matrix (ECM) proteins. Existing methods of manufacturing extracellular matrix protein networks were different from in vivo mechanisms or difficult to control the shape.

This technology utilizes anionic copolymer micropatterns to precisely manufacture an extracellular matrix protein fiber network similar to that in vivo, making it possible to effectively control the shape and arrangement of a single or complex protein network.

This technology contributes to in vitro development, cell adsorption and differentiation research of various tissues, and can be used as a cell culture scaffold essential for the development of biomaterials and medical devices, and as a restorative material for tissue regeneration.

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Key Features:

• Using an anionic copolymer with a micro-pattern, the fiber formation mechanism of extracellular matrix proteins in vivo is precisely simulated
• The anionic copolymer micro-pattern provides negative charges at specific locations to induce the extracellular matrix proteins to self-align and organize, forming a regular fiber network
• Extracellular matrix proteins such as fibronectin and laminin are cultured on the fabricated micro-pattern to form a fiber network that self-assembles along the pattern. Induction
• Complex protein networks can be manufactured by cultivating not only a single protein but also two or more types of proteins sequentially or simultaneously, enabling the simulation of various tissue environments

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This technology was developed through support from the National Research Foundation of Korea's artificial cell structure and function simulation research project.

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90% efficient conversion of carbon dioxide with porous amalgam electrode

This technology concerns the manufacturing method of an amalgam electrode and the electrochemical reduction method of carbon dioxide using an amalgam electrode.

The existing amalgam electrode manufacturing method had difficulties in efficiently converting carbon dioxide due to limitations in implementing porous electrodes, but this technology provides a method of forming an amalgam layer on the surface of a porous substrate electrode by electrodepositing mercury and metal.

This technology overcomes the shortcomings of existing methods and utilizes a large surface area to achieve excellent conversion efficiency of up to 90% or more in the electrochemical reduction reaction of carbon dioxide. It also provides environmental benefits by utilizing safe dental amalgam formulations.

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Key Features:

• Manufacture an amalgam electrode using an electroplating method
• Submerge the substrate electrode in a solution containing metal ions such as mercury, silver, tin, and copper, and sequentially deposit metal layers through an electrochemical reduction reaction
• Applicable to porous substrates, greatly increasing the effective surface area of the electrode
• Stable electrolysis is possible even at high current densities, and efficiency lasts for more than 21 hours

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This technology was developed through support from the Korea Institute of Energy Technology Evaluation and Planning's research project to develop innovative technology for the existing production process of high-value chemical products using captured CO₂.

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Liposome that improves drug delivery efficiency by introducing extracellular matrix (ECM) and cytoskeleton

This technology is about cell-like liposomes. Existing liposomes have limitations in drug delivery due to instability in the body and low delivery efficiency, but it provides cell-like liposomes and an improved drug delivery system using them.

The developed liposome contains an extracellular matrix (ECM) and cytoskeleton in a phospholipid membrane composed of anionic lipids and neutral lipids, so it has strength and flexibility similar to real cells and can dramatically increase drug delivery efficiency by securing high stability and affinity in vivo.

This technology overcomes the shortcomings of existing drug delivery systems and builds a next-generation drug delivery system that is biocompatible and stable, and cell-like liposomes have the characteristics of improved strength and flexibility.

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Key Features:

• Manufacturing a phospholipid membrane composed of anionic lipids, neutral lipids, and ion-permeable carriers
• Introducing a single-molecule cytoskeleton (e.g., G-actin) into the phospholipid membrane through methods such as application of electricity
• Binding the extracellular matrix to the liposome surface through ionic bonding with anionic lipids, and stably coating the surface through self-assembly polymerization reaction
• Thanks to a cell-like structure, it is resistant to changes in the external environment Has resistance

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This technology was developed through support from the National Research Foundation of Korea's artificial cell structure and function simulation research project.

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Conversion of carbon dioxide to oxalate: Eco-friendly system for high purity production

This technology is about an electrochemical conversion system of carbon dioxide in which carbon dioxide is electrochemically reduced to produce oxalate.

Reducing carbon dioxide emissions, the main cause of global warming, is an urgent task, but existing carbon dioxide conversion systems had limitations in terms of efficiency, purity, and environmental friendliness.

This technology is a system that electrochemically converts carbon dioxide into high-purity oxalate, using an amalgam electrode, an aprotic polar organic solvent (DMSO), and a specific By using auxiliary electrolyte (TBA·PF6), high purity oxalate of more than 90% can be produced in an environmentally friendly manner, solving the problems of volatility, explosion risk, and low purity of the existing system.

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Key Features:

• A system that generates oxalate, a high value-added compound, by electrochemically reducing carbon dioxide
• Radicalizes carbon dioxide molecules in an aprotic polar organic solvent environment and then directly combines with other carbon dioxide radicals to form oxalate
• Uses a dental amalgam electrode that is harmless to the human body and has high selectivity for carbon dioxide reduction
• Can be operated at room temperature and pressure, and can be operated at a separate temperature There is no need for a control device or sealing system, so the device construction cost is low and the process is simple

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This technology was developed through support from the Korea Evaluation Institute of Industrial Technology's research project to develop innovative technologies for existing production processes for high value-added chemical products using captured CO2.

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Enzyme for sustained glutathione production without expensive ATP

This technology is about a method of producing glutathione using glutamic acid, cysteine, and glycine as reaction substrates by combining photosynthetic cell membrane vesicles and an enzyme that catalyzes glutathione synthesis.

The existing glutathione production method has the limitation of high production cost due to the problem of continuous supply of expensive adenosine triphosphate (ATP).

This technology is based on photosynthetic cell membrane This is a method of efficiently producing glutathione by continuously reproducing ATP through light energy by combining vesicles and glutathione synthase. It is a method that can dramatically reduce production costs by stably mass producing glutathione without additional ATP input.

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Key Features:

• Using 'photosynthetic cell membrane vesicles' isolated from photosynthetic bacteria or algae
• Glutathione synthesis catalyst enzyme uses the generated ATP as an energy source to synthesize glutathione from glutamic acid, cysteine, and glycine
• ADP and inorganic phosphate generated in the synthesis step go back to step 1 and are regenerated into ATP by light energy and reused in the reaction
• Since it is a reaction using an enzyme, the reaction is selective; Higher yield compared to fermentation method

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Microwave near-field high-resolution imaging microscopy using thermal distribution

This technology is about a microwave near-field imaging microscope. Circularly polarized light is incident on an irradiation object, the reflected light is analyzed, and the heat distribution can be optically imaged using an indicator.

As the demand for wide-area communication systems in the high-frequency band is rapidly increasing, technology for verifying and investigating microwave devices is required, but the existing technology has the disadvantage of long measurement time and difficulty in designing a probe for measurement. There was.

Through this technology, it is possible to investigate the driving method of the device and verify the reliability of the device by providing a microscope that images the microwave near-field by optically imaging the distribution of heat generated by the microwave near-field using an indicator.

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Key Features:

• Combining the 'near-field heating phenomenon', in which a microwave near-field generates heat in a specific material, and the 'photoelastic effect', in which the internal stress changes and the polarization state of light changes when the material is heated
• An indicator is made by depositing a polymer with high dielectric loss when detecting a microwave electric field and a magnetic thin film with high magnetic loss when detecting a magnetic field on a glass substrate with a good photoelastic effect
• The reflected light is split into 45 and 90 degree directions using a spectrometer, and each change in brightness is measured using a CCD camera.

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