The method for production of trans-resveratrol using magnetic photosynthetic membrane vesicle

High purity trans-resveratrol economic production system

This technology is about a method of producing trans-resveratrol, a high value-added product, by using magnetic photosynthetic membrane vesicles together with immobilized biosynthetic enzymes.

Existing trans-resveratrol production methods had limitations such as low yield, complicated purification, and high cost. This technology proposes an innovative in vitro production method using magnetic photosynthetic cell membrane vesicles and immobilized enzymes to increase economic efficiency through repeated reuse of high-cost coenzymes and reduce production costs through efficient recovery and reuse of enzymes. In addition, the final product, trans-resveratrol, can be easily separated in the form of a high-purity precipitate, simplifying the purification process, and providing high-quality resveratrol through stereo-specific production, improving the possibility of industrial mass production of high-value resveratrol.

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Key Features:

• Enzyme-based synthesis: Using 4-coumaric acid and malonic acid as starting materials, trans-resveratrol is synthesized through three major enzymes (4-coumaric acid-Coenzyme A ligase, malonyl Coenzyme A synthetase, and stilbene synthase).
• Coenzyme (ATP) regeneration system: Photosynthetic cell membrane vesicles absorb light energy, continuously converting the ATP consumed during the reaction from ADP. Regeneration
• Magnetic-based recovery: Magnetic particles are contained inside the photosynthetic cell membrane vesicles, and after the reaction, they are simply separated from the reaction solution with a magnet and reused.
• The magnetic photosynthetic cell membrane vesicles regenerate ATP from ADP and Pi using light energy and resupply it to the reaction

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This technology was developed through the cutting-edge convergence technology development research project of the Ministry of Science, ICT and Future Planning.

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