Method for Preparing Fiber Network of Extracellular Proteins and Uses Thereof

Biomimetic fiber network for cell tissue regeneration scaffold

This technology relates to a method of manufacturing a fiber network of extracellular matrix (ECM) proteins. Existing methods of manufacturing extracellular matrix protein networks were different from in vivo mechanisms or difficult to control the shape.

This technology utilizes anionic copolymer micropatterns to precisely manufacture an extracellular matrix protein fiber network similar to that in vivo, making it possible to effectively control the shape and arrangement of a single or complex protein network.

This technology contributes to in vitro development, cell adsorption and differentiation research of various tissues, and can be used as a cell culture scaffold essential for the development of biomaterials and medical devices, and as a restorative material for tissue regeneration.

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Key Features:

• Using an anionic copolymer with a micro-pattern, the fiber formation mechanism of extracellular matrix proteins in vivo is precisely simulated
• The anionic copolymer micro-pattern provides negative charges at specific locations to induce the extracellular matrix proteins to self-align and organize, forming a regular fiber network
• Extracellular matrix proteins such as fibronectin and laminin are cultured on the fabricated micro-pattern to form a fiber network that self-assembles along the pattern. Induction
• Complex protein networks can be manufactured by cultivating not only a single protein but also two or more types of proteins sequentially or simultaneously, enabling the simulation of various tissue environments

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This technology was developed through support from the National Research Foundation of Korea's artificial cell structure and function simulation research project.

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