Recombinant Pseudomonas putida Strain and Method for Producing Mevalonate Using the Same

High-Concentration Ethanol-Based Mevalonate Production Using Recombinant Pseudomonas putida

This technology utilizes a recombinant strain of Pseudomonas putida engineered with a mevalonate biosynthetic pathway by introducing a recombinant vector containing acetyl-CoA acetyltransferase and HMG-CoA synthase/reductase genes, while deleting endonuclease A and X genes.

While mevalonate is a key precursor for terpenoids and a raw material for cosmetics and bioplastics, high-concentration production has been difficult when using sugars as a carbon source due to byproducts from glycolytic intermediates. Furthermore, terpenoids derived from mevalonate are non-polar hydrocarbons with high microbial toxicity, complicating fermentation process development.

This technology enhances the stability of introduced genes and regulates ethanol catabolism and fatty acid biosynthetic pathways to significantly increase mevalonate yield using ethanol as a carbon source. As the host possesses high resistance to organic solvent toxicity, it can be applied to biocatalysts for terpenoid fermentation and bioplastic raw material processes, bypassing the byproduct limitations of sugar-based processes.

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Key Features:

• A strain transformed with a recombinant vector containing nucleic acid sequences for acetyl-CoA acetyltransferase, HMG-CoA synthase, and HMG-CoA reductase
• A recombinant Pseudomonas putida strain with deleted endonuclease A and endonuclease X genes
• A strain with additional deletions of quinoprotein ethanol dehydrogenase I and II genes, further containing an acetyl-CoA synthetase nucleic acid sequence
• A method for producing mevalonate by culturing the recombinant Pseudomonas putida strain and recovering mevalonate from the culture product

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This invention was developed with support from the Ministry of Science and ICT's C1 Gas Conversion Technology common research project.

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