This technology is a bioconversion production method that obtains 10-hydroxystearic acid by converting oleic acid using recombinant E. coli that expresses oleate hydratase derived from Stenotrophomonas maltophilia. It specifies the timing for inducing enzyme expression and adding the substrate, tailored for both flask and bioreactor cultivation.
Existing production strains, such as Flavobacterium or Nocardia cholesteroliphagum, have low yields and produce byproducts like 10-ketostearic acid, making them uneconomical for industrialization. There have been no previously reported cases of production using enzymes or recombinant microorganisms containing related enzymes.
This technology expresses highly substrate-specific oleate hydratase in recombinant microorganisms to reduce byproduct formation and ensure high yields in an eco-friendly manner. As the product is a starting material for lactone synthesis, it can be applied to the manufacturing of natural fragrances, cosmetic ingredients, and fine chemical raw materials, providing a supply route for naturally derived ingredients that can replace petrochemical synthesis.
This invention was developed with support from the Small and Medium Business Administration for the development of natural hydroxy fatty acid and lactone production processes.
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