This technology involves mixing a solution containing extracellular vesicles or cells with poly(acrylamide-co-acrylic acid) superabsorbent polymers and incubating them to immobilize the vesicles or cells onto the polymer surface. Resuspending the immobilized polymer in a different solvent allows for simultaneous solvent exchange and sample recovery.
Extracellular vesicles in bodily fluids or culture media are typically found at low concentrations, requiring separate concentration and isolation processes. Size-exclusion chromatography often dilutes the sample during elution, while polyethylene glycol precipitation risks leaving impurities that can alter vesicle properties. Ultracentrifugation suffers from low yields and potential damage to vesicles due to high centrifugal forces.
This technology enables direct biomarker detection while vesicles are captured on the resin bead surface and allows for easy solvent exchange into PBS or culture media without contamination from the previous solvent. Applicable to the development of diagnostic compositions and kits for diseases like cancer, as well as pharmaceutical, cosmetic, and food ingredients, it simplifies vesicle pretreatment without the need for expensive ultracentrifugation equipment.
This invention was developed with support from the Ministry of Science and ICT for the development of production processes and engineering platform technology for plant-derived extracellular vesicles.
WO2024-075941A1
