Pseudomonas azotoformans strain KACC 92125P and compositions containing it

Pseudomonas antagonist strain that inhibits both Staphylococcus aureus and aflatoxin-producing fungi

This technology features the Pseudomonas azotoformans KACC 92125P strain isolated from food, along with antimicrobial compositions, feed additives, and biological control agents containing its cultures, extracts, or suspensions. It also includes agents for controlling ear rot in grains and methods for surface antimicrobial treatment.

Staphylococcus aureus is resistant to desiccation, causing cross-contamination and food poisoning on food processing surfaces, while Aspergillus flavus produces the carcinogen aflatoxin, contaminating grains and feed, leading to immune suppression, stunted growth, and livestock mortality. Chemical disinfectants pose health risks to workers, and it has been difficult to find microbial agents that simultaneously inhibit bacteria and toxin-producing fungi while persisting on surfaces.

This technology uses a single strain to simultaneously inhibit Gram-positive foodborne pathogens and toxin-producing fungi, maintaining antagonistic activity by forming biofilms even on dry surfaces. It is also used as a control agent applied directly to plants to target Aspergillus flavus, which causes ear rot in crops like corn. Applicable to fungal disease control in grain fields, preventing aflatoxin contamination in feed, and sanitizing food processing surfaces, it allows for the management of mycotoxins and foodborne pathogens from the field to the feed and food factory using a single biological agent.

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Key Features:

• Pseudomonas azotoformans strain deposited under accession number KACC 92125P, and its cultures, extracts, or suspensions. Antimicrobial compositions containing the strain as an active ingredient to simultaneously inhibit Staphylococcus aureus and Aspergillus flavus. Control agents containing the strain for application to plants to prevent ear rot caused by Aspergillus flavus. Surface antimicrobial treatment steps involving spraying the strain onto surfaces such as stainless steel, glass, plastic, or tile, followed by incubation at 20°C to 30°C.

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This invention was developed with support from the Ministry of Agriculture, Food and Rural Affairs for the development of seed sterilization technology for healthy seed production.

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