This technology features a novel Pseudomonas veronii strain isolated from lettuce, along with compositions, feed additives, and biocontrol agents containing the strain or its cultures, extracts, or suspensions for inhibiting enterohemorrhagic E. coli O157:H7. It also includes treatment methods for suppressing pathogenic E. coli activity by applying the strain to livestock, barns, or food-contact surfaces.
Enterohemorrhagic E. coli O157:H7 produces verotoxin, causing severe illnesses like hemolytic uremic syndrome, and frequently cross-contaminates food via various surfaces in livestock, transport, and slaughter environments. While chemical disinfectants like hypochlorite, chlorine dioxide, and ethanol are effective, they carry side effects such as dermatitis and toxic residue. Microbial or biofilm-based approaches to control foodborne pathogens on environmental surfaces remain largely undeveloped.
This technology enables the antagonistic strain to form biofilms on surfaces like stainless steel, glass, wood, plastic, rubber, and tile, even in dry conditions, providing sustained antimicrobial effects from a single application. It can be formulated as live cells, dead cells, or dried products. Applicable to food processing surface hygiene, livestock and slaughterhouse pathogen control, and feed additives, it reduces chemical disinfectant use and breaks the cross-contamination chain from farm to table.
This invention was developed with support from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry for the development of seed disinfection technology for the production of healthy and vigorous seeds.
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