This technology provides a composition, coating composition, and inhibition method that uses the FDA-approved histone deacetylase inhibitor SAHA (vorinostat) as an active ingredient to suppress biofilm formation by Salmonella species. The composition can be applied or coated onto food surfaces such as chicken skin and eggshells, food-contact surfaces like glass, rubber, silicone, and plastic, and medical devices.
Salmonella is a zoonotic pathogen that causes sepsis, diarrhea, and pneumonia, with increasing rates of antibiotic resistance. It forms biofilms on the surfaces of medical devices and food processing equipment made of stainless steel, plastic, and rubber, making it difficult to remove even with antimicrobial agents. Treatments such as electric fields, ultrasound, and high pressure have shown minimal sterilization effects, are limited in application area, and are costly. Furthermore, acid-base cleaning solutions and chlorine-based disinfectants cause metal corrosion, produce carcinogens like trihalomethanes, and pose risks of recontamination due to the reuse of wash water.
This technology utilizes SAHA at a minimum inhibitory concentration of 120 μg/mL to inhibit the swimming and swarming motility of S. enterica and downregulates the expression of genes related to pathogenicity and quorum sensing, preventing biofilm establishment. It can be applied to surface hygiene management in poultry processing and egg grading plants, coating rubber and silicone parts in food processing equipment, and preventing infections on medical implants like catheters. This allows for blocking the attachment phase of Salmonella using a drug with proven safety, eliminating the need for corrosive disinfectants or large volumes of wash water.
This invention was developed with research support from the Ministry of Science and ICT for identifying the causes of pathogenic bacterial biofilm formation in the food industry and developing efficient control technologies that combine physical, chemical, and biological techniques.
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