Embryonated egg-derived cell growth-promoting composition for cultured meat production and manufacturing method thereof

Cell growth medium for cultured meat that reduces FBS dependency using sterilized extract from embryo-removed embryonated eggs

This technology involves a cell growth-promoting composition and its manufacturing method, created by mixing a sterilized liquid—obtained by removing embryos from 8–12 day embryonated eggs, followed by homogenization, centrifugation, filtration, and sterilization—with serum-free medium and FBS in an 8:1:1 volume ratio. This medium is used to culture and differentiate livestock muscle satellite cells for cultured meat production.

Fetal Bovine Serum (FBS), rich in growth factors, has been a key nutrient in cultured meat media. However, because FBS is obtained by slaughtering pregnant cows to collect fetal blood, it raises significant animal welfare and ethical concerns. Limited supply also drives up production costs, and the paradox of increasing slaughter to produce cultured meat necessitates alternatives that minimize serum use.

This technology utilizes embryonated eggs, where embryos accumulate abundant nutrients and growth factors in the yolk and albumen, as a raw material. Extracts are obtained via ultrasonic homogenization and dilution of the supernatant, then combined with Ham's F-10 serum-free medium. In chicken myoblast cultures, this showed higher growth rates than media containing only FBS; antibiotic mixtures and bFGF can also be added. Applicable to pork, beef, and poultry cultured meat, commercial media production, and muscle cell research, it uses inexpensive embryonated eggs to reduce FBS reliance and establish a foundation for slaughter-free mass production.

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Key Features:

• Step of obtaining embryo-removed embryonated eggs by removing embryos from 8 to 12-day embryonated eggs
• Step of filtering and sterilizing a diluted filtrate of the supernatant obtained by ultrasonically homogenizing and centrifuging the embryo-removed embryonated eggs
• Step of obtaining a composition that yields higher cell growth rates than FBS alone by mixing Ham's F-10 serum-free medium, the sterilized liquid, and FBS in an 8:1:1 volume ratio
• Step of producing cultured meat by culturing muscle satellite cells isolated from livestock muscle tissue in the composition-containing medium for 3 days, followed by differentiation into myocytes and myotubes

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This invention was developed with support from the Ministry of Agriculture, Food and Rural Affairs for the development of optimal muscle tissue engineering and mass production systems for cultured meat.

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