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IBL-26-0666

Water Toxicity Assessment Method Using Root Length of Duckweed Germinated from Turions

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2026-10-28
Water Toxicity Assessment Method Based on Root Length Measurement of Germinated Duckweed Turions

This technology quantitatively assesses water toxicity by placing water samples into a culture vessel, introducing dormant duckweed turions to germinate, and measuring the length of the newly grown roots. It compares concentration-dependent responses by testing diluted raw water at various dilution ratios alongside a non-toxic control medium.

Previous methods, such as directly measuring pollutant concentrations in organisms or identifying the presence of specific species, struggled to quantify pollution levels. Furthermore, ecotoxicity tests using animals or microalgae often required specialized expertise, long incubation periods, and lacked sufficient sensitivity.

This technology uses a single numerical indicator—root length—to identify the effects of heavy metals like silver, cadmium, mercury, and lead, as well as organic toxins like acetone, phenol, and formalin, within approximately three days. By using a dilution series created through half-dilution, it can also determine the concentration-dependency of toxicity. Applicable to public water monitoring networks, wastewater treatment plant effluent management, and environmental risk assessments, it allows for the numerical comparison of complex toxicity from unknown sources without the need for instrumental analysis.

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Key Features:
  • A step of placing a water sample into a culture vessel and introducing dormant turions separated from duckweed
  • A step of incubating the introduced turions to germinate and measuring the root length of the resulting duckweed
  • A step of preparing water samples by half-diluting raw water and at least two other diluted raw water samples with different dilution ratios using a turion culture medium
  • A step of testing a culture medium free of toxic substances that inhibit turion germination as a control group for the raw water

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This invention was developed through the Ministry of Trade, Industry and Energy's project for the development of standard technologies for aquatic ecological risk bio-diagnosis and support for international certification.

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Incheon National University
Tae-Jun Han | Ji-Hye Park
Document
Date of application:
2014-03-25
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Patent registration number:
10-1614923
Industry
environment•eco
Technology
Bio/Pharmaceutical
Country
Korea
Family Patent

N/A

Price
4000000
Fixed price
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