New Pleurotus eryngii strain DDL01 and its cultivation method

Cultivation method for Pleurotus eryngii using high-pressure sterilized substrate and multi-stage incubation and primordia formation

This technology involves a cultivation method for the Pleurotus eryngii DDL01 strain, which includes adjusting the moisture content of a sawdust and rice bran substrate, high-pressure sterilization, spawn inoculation, and subsequent stages of early/late incubation, mycelium scraping, primordia formation, and growth.

Stable cultivation of Pleurotus eryngii requires precise control over substrate composition and incubation/primordia formation conditions, which has historically been difficult to maintain consistently.

By defining specific parameters for substrate moisture, sterilization, and multi-stage incubation, primordia formation, and growth, this technology enables the stable production of Pleurotus eryngii DDL01 in commercial cultivation processes.

‍

‍

Key Features:

• Substrate preparation stage: Mixing sawdust and rice bran to adjust the final substrate moisture content to 68–70%.
• Substrate sterilization and inoculation stage: High-pressure sterilization of the prepared substrate at 120°C for 60 minutes, followed by spawn inoculation.
• Incubation stage: A two-stage process involving early and late incubation with distinct temperature and humidity settings.
• Primordia formation and growth stage: Growing young mushrooms through early and late primordia formation stages following mycelium scraping.

‍

This patent is owned by IPBank. Upon purchase, you may receive support through commercialization programs, recommendations for technology transfer and commercialization funding guarantees, and additional points during evaluations for priority procurement of excellent inventions.

‍