Perfusable Dual Proximal Tubular Cell Construct for Use in In Vitro Artificial Organ Models and Cell Therapy, and Method for Preparing the Same

Perfusable Dual Proximal Tubular Cell Construct and Method for Preparing the Same

This technology describes a perfusable proximal tubular cell construct, printed co-axially and dual-tubularly using two types of bio-ink containing kidney tissue-derived decellularized material and cells, and its method of production.

Existing cell-based artificial kidney tissues and their production methods have had limitations in terms of maturation and functionality.

This technology matures a dual tubular structure, comprising vascular endothelial cells and proximal tubular epithelial cells, in a perfusion environment. It is then utilized as a bioreactor capable of observing drug responses similar to those in vivo.

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Key Features:

• Bio-ink based on kidney tissue-derived decellularized material
• Inclusion of vascular endothelial cells and proximal tubular epithelial cells
• 3D printing of co-axial dual tubular structure
• Tissue maturation of perfusion-based artificial proximal tubule chip