This technology is a composition for detecting mercury ions that includes a resorufin derivative with a phosphinothioate group, which reacts with mercury ions to form a resorufin derivative salt. It covers the detection method using the resulting colorimetric and fluorescent signals, as well as the manufacturing process for the detection compound.
Because mercury bioaccumulates in the food chain and damages the kidneys, brain, endocrine system, and nervous system, there is a critical need to selectively quantify trace amounts in both environmental and biological samples. However, existing mercury analysis methods require expensive equipment, time-consuming sample preparation, and skilled personnel, making on-site testing difficult.
This technology uses a structure where mercury ions induce desulfurization of the phosphinothioate group, revealing a fluorogenic moiety that produces simultaneous color changes and turn-on fluorescence. It distinguishes mercury using a ratiometric approach, even in the presence of other coexisting metal ions and anions. Measurements are performed simply by mixing in buffer solution and organic solvent conditions. It can be applied to water and soil environmental monitoring, urine-based mercury exposure assessment, and workplace safety inspections, allowing for the development of on-site rapid diagnostic kits.
N/A
