This technology is an electrochemical aptasensor featuring a working electrode surface-modified with a composite of graphene nanoplatelets, transition metal dichalcogenides, and amine-based polymers. Gold nanoflowers are grown on this surface, and the aptamer PT01, which binds to the plasticizer DEHP, is immobilized. The technology also includes the sensor manufacturing method and a target substance analysis method using the sensor.
DEHP accounts for a significant portion of the global plasticizer market, but as an endocrine disruptor capable of causing cancer and metabolic disorders, it requires trace-level monitoring. While electrochemical methods offer advantages in sensitivity and ease of operation, existing sensors have lacked sufficiently low detection limits and the sensitivity required to easily monitor the trace amounts leaching from actual plastic products.
This technology increases the electrode surface area and electron transfer through a nanocomposite layer and electrodeposited flower-shaped gold structures, enhancing aptamer immobilization density. Thiol-based molecules are used to block remaining sites, reducing non-specific signals. As a result, it achieves a low detection limit with simple measurements. It can be used for on-site analysis, such as testing plasticizer leaching in food packaging, plastic toys, and cosmetic containers, or for monitoring environmental samples.
This invention was developed with support from the Ministry of Environment's project for developing direct-reading measurement technology for emerging contaminants entering aquatic ecosystems.
US11933755B2
