This technology features an ultra-thermostable enzyme created by attaching a Strep-tag to the C-terminus of methylglyoxal synthase derived from the deep-sea hyperthermophilic microorganism Oceanithermus profundus DSM 14977. It includes the enzyme itself, the gene encoding it, recombinant vectors, transformed microorganisms, and a method for producing methylglyoxal using the enzyme.
Most 1,2-propanediol is produced via petrochemical processes, which generate harmful intermediates and byproducts. Replacing this with a bio-based process requires an efficient enzyme to produce the key intermediate, methylglyoxal. However, there is a lack of activity data for enzymes derived from sources other than E. coli and B. subtilis, limiting the options for enzymes suitable for high-temperature processes.
This technology maintains activity across a wide temperature range from 30°C to over 100°C and exhibits a specific activity approximately 4.9 times higher than that of the enzyme derived from the thermophile Thermus sp. GH5. The C-terminal Strep-tag allows for easy isolation and purification of the enzyme. It can be applied to high-temperature, bio-based 1,2-propanediol and methylglyoxal production processes, ensuring high production efficiency in low-contamination high-temperature reactors.
This invention was developed with support from the Ministry of Science and ICT's C1 Gas Conversion Technology common research project.
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