This technology involves isolating the demethylation-related NtROS2a gene from tobacco leaves, introducing it into plant cells via a recombinant vector for overexpression, and regenerating the cells to create transgenic plants resistant to drought, oxidation, and aluminum stress. It also includes the transgenic plants, seeds, and compositions for increasing stress tolerance containing the gene as an active ingredient.
Drought stress disrupts osmotic pressure and ion balance within plant cells, reducing growth and photosynthesis, while soluble aluminum in acidic soil inhibits root growth and the uptake of nutrients and water, significantly limiting agricultural productivity. Although various stress-inducible genes have been reported, the role of NtROS2a, which contains a DNA glycosylase domain, in environmental stress tolerance had not been previously identified.
This technology enables NtROS2a-overexpressing plants to exhibit longer root growth under drought conditions, maintain higher chlorophyll content under oxidative conditions, and show significantly increased shoot, root, and lateral root growth under aluminum stress. A key feature is the ability of a single gene to simultaneously address multiple types of abiotic stress. It can be applied to breeding drought-tolerant crops for arid regions, developing aluminum-tolerant varieties for acidic soil cultivation, and producing biofuel crops in challenging conditions, securing GM crop traits that maintain yields in poor soil and drought environments.
This invention was developed with support from the Rural Development Administration for the breeding of multi-disease resistant GM event lines using antimicrobial peptides.
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