Composition for inhibiting ER stress-induced apoptosis comprising silkworm storage protein 1 as an active ingredient

Composition for Inhibiting Endoplasmic Reticulum Stress-Induced Apoptosis Based on Silkworm Hemolymph-Derived Storage Protein 1

This technology utilizes Storage Protein 1 (SP1), isolated from silkworm hemolymph, as an active ingredient to inhibit endoplasmic reticulum (ER) stress and subsequent apoptosis caused by the release of calcium into the cytoplasm.

The endoplasmic reticulum is responsible for protein folding, calcium storage, and signal transduction. When dysfunction occurs due to conditions such as abnormal calcium concentrations or increased protein synthesis, it leads to apoptosis. Although this ER stress is associated with various diseases, including diabetes, amyloidosis, and cystic fibrosis, there has been a lack of naturally derived materials capable of effectively mitigating it.

This technology mitigates ER stress by having SP1 reduce the release of calcium from the ER into the cytoplasm, lower the expression of stress markers CHOP and GRP78, and influence the expression of apoptosis-related proteins. This material can be applied to the development of reagents for research into ER stress-related diseases and cytoprotective agents, offering a new high-value application pathway for silkworm-derived proteins.

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Key Features:

• A composition comprising, as an active ingredient, Storage Protein 1 isolated from silkworm hemolymph and having the amino acid sequence of SEQ ID NO: 1
• A composition that inhibits apoptosis caused by endoplasmic reticulum stress induced by the release of calcium ions into the cytoplasm in vitro
• A composition in which Storage Protein 1 inhibits apoptosis caused by endoplasmic reticulum stress induced by increased expression of CHOP and GRP78 genes
• A composition for inhibiting apoptosis caused by endoplasmic reticulum stress induced by blocking disulfide bond formation of proteins within the endoplasmic reticulum

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