This technology provides a culture medium composition for preventing apoptosis that includes exosomes extracted from CHO cell culture fluid along with a basal medium, as well as a culture method that reuses exosomes obtained from the same cells.
CHO cells used in biopharmaceutical production often experience apoptosis due to various stresses during fed-batch culture, leading to a decline in both viable cell concentration and product quality. Methods to inhibit apoptosis through external additives or genetic modification have faced limitations in practical process application due to concerns regarding human safety, high costs, and efficiency.
By using exosomes derived from the cells themselves as a medium component, this technology inhibits cell activity decline and apoptosis without concerns about toxicity or side effects, thereby increasing viability. It can be applied to large-scale production processes for CHO-based biopharmaceuticals, such as monoclonal antibodies, improving both culture yield and product quality without the need for additional additives.
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