This technology is a biosensor that detects target substances by immobilizing target-specific aptamers on a graphene oxide-modified electrode and electrochemically reducing the graphene oxide after target binding to measure the shift in reduction potential. It utilizes graphene oxide itself as a label-free electrochemical indicator.
Hirudin-based immunoassays, used to measure biomarkers like thrombin where abnormal blood concentrations indicate disease, have failed to detect thrombin bound to other proteins. Widely used ELISA methods require careful storage due to unstable protein reagents and rely on optical analysis, which necessitates expensive equipment.
This technology leverages the phenomenon where the reduction peak potential of graphene oxide shifts in the positive direction as the target concentration increases, allowing for high-sensitivity and high-selectivity analysis without additional redox labels. By simply changing the aptamer sequence, the target can be expanded to include metal ions, DNA, ATP, and various proteins. This enables the creation of a versatile, low-cost electrochemical detection platform for disease diagnosis, food analysis, and portable environmental monitoring, eliminating the need for expensive optical equipment.
This invention was developed with support from the Ministry of Education for the development of ultra-sensitive target detection methods using thrombin enzymatic reactions.
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