Graphene thin film obtained by electrochemical reduction of hydroxyl-rich graphene oxide, and uric acid detection method using the same

Highly Selective Uric Acid Detection Kit Based on 10-Second Electrochemically Reduced Graphene Thin Film

This technology provides a method for manufacturing a uric acid detection kit and a method for detecting uric acid using it. The kit is produced by coating an ITO electrode pretreated with PEI with hydroxyl-rich graphene oxide (HGO) and electrochemically reducing it in an electrolyte solution to form an ERHGO thin film. It utilizes a holding reduction method, where the reduction potential is maintained at the peak point for a short duration before the supply is cut off.

Conventional graphene oxide is difficult to fix onto electrodes, making it hard to create a uniform and stable surface. Furthermore, its lack of electrochemical catalytic activity has limited its use in detecting bodily substances like uric acid. Chemically reduced graphene oxide also faces issues with unwanted byproducts resulting from the use of reducing agents such as hydrazine or NaBH4.

This technology uses HGO to stably fix more graphene oxide onto the electrode and creates a clean, byproduct-free detection surface by electrochemically reducing it in an NaCl aqueous solution in about 10 seconds without reducing agents. By separating oxidation peaks even in samples containing both uric acid and ascorbic acid, it can be applied to point-of-care gout diagnostic devices, kidney disease monitoring tools, and home uric acid meters, providing reliable readings even in real bodily fluids with many interfering substances.

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Key Features:

• Pretreating a cleaned ITO electrode by dipping it in a PEI solution, followed by coating the electrode surface by dipping it in a hydroxyl-rich graphene oxide solution
• Preparing hydroxyl-rich graphene oxide by adding potassium persulfate to a graphene oxide solution, heating it, and dialyzing it to a pH of 3 to 4
• Electrochemically reducing the coated hydroxyl-rich graphene oxide in an NaCl electrolyte solution by maintaining the reduction potential peak point for 10 seconds
• Quantifying uric acid by applying a sample containing both uric acid and ascorbic acid to the kit electrode and obtaining oxidation peaks separated by oxidation potential

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