This technology is an electrochemical detection method and kit that quantifies thrombin by mixing ferrocene-labeled fibrinogen with a sample, applying it to an electrode kit coated with a fibrinogen layer, and measuring the redox current. It utilizes the enzymatic reaction where thrombin converts fibrinogen into fibrin as the signal generation principle.
Existing immunoassays using hirudin peptides struggle with accurate quantification because hirudin cannot bind to thrombin if it is already bound to other proteins. Aptamer-based methods require immobilizing aptamers on a surface, which leads to reduced protein binding efficiency due to surface interference and a decrease in protein activity.
This technology avoids immobilizing proteins or aptamers on the electrode surface, instead aggregating ferrocene-labeled fibrinogen on the electrode via an enzymatic reaction in solution. This allows for rapid, accurate thrombin quantification without compromising protein activity, achieving higher detection efficiency than aptamer-based methods. Applicable to coagulation tests, thrombosis diagnostic kits, and anticoagulant therapy monitoring, it enables on-site blood thrombin activity checks using only a simple electrode kit.
This invention was developed with support from the Ministry of Science, ICT and Future Planning for the development of high-sensitivity target detection methods using thrombin enzymatic reactions.
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