Establishment of a Cell Surface Expression System Utilizing Autotransporters

Cell Surface Expression System Utilizing Autotransporter Proteins

This technology concerns the establishment of a system that utilizes autotransporter (self-transporter) proteins to express target proteins on the cell surface.

Existing cell surface expression methods have been limited in terms of expression targets and efficiency, thereby restricting their application in various genetic engineering fields.

This technology provides a surface expression vector with a cloning site for the fusion of target proteins and scaffold proteins (YfaL autotransporter), enabling efficient expression of target proteins on the cell surface.

‍

‍

Key Features:

• Utilizes autotransporter (YfaL) protein as a scaffold
• Surface expression vector with a cloning site for target protein fusion
• Efficient expression of target proteins on the cell surface
• Expression system applicable to various genetic engineering applications