This technology relates to a microdroplet-based microchip for digital recombinant enzyme-polymerase isothermal amplification and an isothermal amplification method using the same. In particular, it is a technology designed to enhance performance, durability, stability, and applicability based on core materials, structures, processes, or device configurations related to the microdroplet-based digital recombinant enzyme-polymerase isothermal amplification microchip and the isothermal amplification method using the same.
To address the limitations of existing recombinant enzyme-polymerase isothermal amplification methods, such as false positives and false negatives, by providing a microchip that forms high-efficiency microdroplets for low error rates and high-accuracy amplification, this technology applies a microchip having primary, branching, secondary, tertiary, junction, and quaternary channels as a core means for generating and manipulating microdroplets for isothermal amplification, and proposes a technical concept for forming high-efficiency microdroplets for low error rates and high-accuracy amplification using the microchip.
Accordingly, the present invention is expected to improve the sensitivity and accuracy of isothermal amplification reactions by providing a microchip that forms high-efficiency microdroplets for low error rates and high accuracy amplification, while simultaneously enhancing reproducibility, scalability, and process suitability in real-world usage environments. Furthermore, since it can be utilized in related industries as a high-performance material, device, battery, sensor, apparatus, or manufacturing process, it is advantageous in terms of subsequent commercialization and demonstration development.
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