Recombinant Pinctada fucata Pif97 protein and composition comprising the same

준Composition that induces the formation of metastable calcium carbonate crystals

This technology relates to the recombinant pearl shell Pif97 protein and the composition for forming metastable calcium carbonate crystals.

Pearls are not only valuable as jewelry, but the fracture resistance of nacre aragonite has a mechanical strength 3,000 times higher than that of pure aragonite, but there is a problem in that it is difficult to synthesize metastable calcium carbonate crystals to make artificially. In order to solve this problem, this technology proposes a method in which the recombinant pearl shell Pif97 protein, represented by SEQ ID NO: 1, produced by recombinant pearl shell Pif97 protein in prokaryotic cells, has the ability to bind calcium, aragonite, and chitin and induces the formation of metastable calcium carbonate crystals.  

The recombinant pearl shell Pif97 protein according to the present technology has the ability to bind calcium, aragonite, and chitin, and is excellent in inducing the formation of metastable calcium carbonate crystals by stabilizing amorphous calcium carbonate in an unstable state and inhibiting the formation of stable calcite.

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Key Features:

• * Providing isolated recombinant pearl shell Pif97 protein, synthesized by the nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO: 1
• * Produce and purify the recombinant protein rPif97 from the prepared vector, and then transform E. coli BL21 (DE3) (Novagen) with the recombinant plasmid pET23-rPif97
• * 1mM Induction of recombinant protein rPif97 expression using IPTG (isopropyl-β-D-thiogalactopyranoside; Sigma Aldrich)
• * The eluate was desalted using a PD-10 column using 10mM Tris buffer (pH8)}
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This technology was developed through support from the Korea Institute for Ocean Science and Technology Promotion's research projects on marine fiber composite materials and bioplastic materials.

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