Chimeric carbonic anhydrase from Dunaliella Salina

High CO2 hydration activity carbonic anhydrase'

This technology is about chimeric carbonic anhydrase derived from Dunaliella salina.

When trying to apply protein as a biocatalyst for removal and conversion of existing carbon dioxide, the low yield of Dsp-CA-c became a problem. To solve this problem, we propose carbonic anhydrase [Dsp-nCA-c] containing the amino acid sequence shown in SEQ ID NO: 4. The chimeric proteins Dsp-nCA-c and Dsp-nCA-c (G263S) of the invention show higher water-soluble expression and CO2 hydration activity than the existing Dsp-CA-c. The two proteins of this technology show increased soluble expression and CO2 hydration activity in Dsp-CA and CO2 mineralization, so they can be used in the CO2 removal process using CA catalysts and the production of various industrial raw materials using CO2 mineralization. They can also be used as catalysts in the synthesis of useful metabolites to increase synthesis yield.

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Key Features:

• Carbonic anhydrase containing the amino acid sequence shown in SEQ ID NO: 4 [Dsp-nCA-c]
• Carbonic anhydrase containing the amino acid sequence shown in SEQ ID NO: 5 [Dsp-nCA-c(G263S)]
• Capture of carbon dioxide containing carbonic anhydrase or Fixation
• Increase the amount of soluble expression by fusing part of the amino terminus to a protein with low soluble expression, such as Dsp-CA-c.

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This technology was developed through research support from the Korea Institute for Ocean Science and Technology Promotion for the development of marine silica biomineral-based synthetic bone graft materials.

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