Composition of Fluorescent proteins for analyzing DNA sequences and method for analyzing DNA sequences

Sequencing technology for optical identification of single DNA

This technology relates to a composition for DNA sequence analysis and a DNA sequence analysis method, and to a DNA sequence analysis method including a composition containing a DNA-binding fluorescent protein and steps for processing the same.

Existing DNA sequence analysis technology had limitations in DNA damage and information loss. To solve these problems, this technology provides a fluorescent protein composition and method to precisely analyze DNA sequences at the level of a single DNA molecule.

By using A/T-specific DNA binding protein and non-specific complementary DNA binding protein simultaneously, efficient optical identification of single DNA molecules is possible without a separate sequencing procedure. This technology is useful for studying chromosomal organization and protein immunoorganization of the genome, and can improve the accuracy and efficiency of genetic analysis.

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Key Features:

• Principle of simultaneously using two types of DNA-binding proteins bound to fluorescent proteins of different colors
• A/T-specific DNA-binding protein preferentially binds to A/T-rich regions of DNA
• A/T non-specific complementary DNA-binding protein binds to the remaining DNA backbone and stains the entire DNA
• A sequence-specific two-color fluorescent pattern, or 'barcode', is generated on the DNA molecule, enabling optical identification without separate sequencing. possible

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This technology was developed through support from the National Research Foundation of Korea's research project on microfluidic devices for observing the structure of dyed yarns for detecting DNA damage.

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